At a minimum, biosafety cabinets shall be used for BSL-2 work unless the IBC permits benchtop work, and when the need arises for manipulation of pathogens that are likely to create aerosols (such as vortexing open tubes, pipetting, opening caps after centrifuging, sonicating, aspirating with a syringe, etc.). Use BSCs for all manipulation of airborne transmitted pathogens.
Working in a BSC
The work station within the BSC should be set up such that work is conducted from "clean to dirty." That means that stock solutions, clean serological pipets, etc., should be on one side of the cabinet, your working area in the middle and used media, serological pipets, etc. collected on the side opposite the clean side.
Aspiration of culture media with a vacuum should be set up as follows:
This setup can be right to left or left to right, but on the "dirty" side of the biological safety cabinet.
How do I maintain my BSC?
一道本不卡免费高清Wipe down surfaces with 70 percent ethanol or other appropriate disinfectant before and after use.
If using the internal UV lamp as part of the sterilization process, be sure to wipe down the lamp with 70 percent ethanol once a week.
Biosafety cabinets require annual certification if you are using infectious agents, human cell lines or tissues and before relocation of the cabinet. The BSC must be re-certified again一道本不卡免费高清 after it has been moved to ensure proper function.
Certification of BSCs is not一道本不卡免费高清 conducted by EH&S, but the biosafety officer participates in the regulation of BSC use based on certification status. PIs are responsible for having their BSCs certified after purchase, followed by yearly certification.
Note that work shall not be conducted in BSCs that are out of date or have expired certification.